The PRO-SPER project approach is to develop integrated, flexible and interconnected processes that transform a range of agro-food by-products into bio-products required by the market - fig. 1.

Fig. 1. The approach of the PRO-SPER complex project of closing the value chains from the bioeconomy by using the nano-bio-technological processes for the transformation of some by-products into the bio-products required by the market

            This approach facilitates the achievement of the goal of the PRO-SPER complex project, coordinating and correlating the research activities of the member organizations of the consortium, INCDCP-ICECHIM, UDJ, ICMPP, INCDSB and UAV, to improve the institutional performance in the field of nano-bio-technologies and their applications in the bioeconomy.

            The overall objective of the PRO-SPER project is to increase the impact of R & D and innovation activities of RDI institutions in the consortium by developing and capitalizing innovative technological solutions for bio-nano-processing of by-products of bioeconomic value chains for recovery and / or the formation of added-value components and their use for high added-value bioproducts.

The specific objectives of the PRO-SPER project are:

• Implement a common R & D and innovation agenda to synergize synergies and resources at the consortium level to provide innovative solutions for closing the value chains of the bio-economy;
• Consolidation of the components of RDI institutions by granting checks to capitalize on the research infrastructure and the human resource and to transfer knowledge in the field of bioeconomics;
• Development of a long-term partnership for the provision of scientific and technological services in the priority field of the circular bio-economy and for mutually beneficial collaboration with the economic agents, including for the joint exploitation of the results of the project;
• Joint participation in joint European project competitions (H2020, JU-BBI) and trans-national (ERA.NET, Eurostar / Eureka, InterReg IV), at least 2 partners in the consortium, to scale up developed technologies up to TRL4 level through component projects and / or capitalizing on newly acquired knowledge;
• Promoting excellence and smart specialization by coagulating niche areas at the intersection of common nano-bio-technology concerns and synergistic integration of several interconnected innovative solutions;
• Broadly disseminate the knowledge and research results obtained within the PRO-SPER project to ensure their superior valorisation

Project 1 "Developing a new generation of biostimulants for plants by capitalizing the exhausted substrate from Pleurotus - NeXT-US lignocellulosic culture"
The NeXT-US project aims to develop a bioproduct from the new generation of biostimulants for plants. The specific objectives of the NeXT-US project are: (i) extracting the components of interest and converting them into active ingredients; (ii) obtaining the new generation biostimulant bioproduct through nanoformulation and dispersive conditioning; (iii) verifying the biological activity of the new biostimulant bioproduct under controlled and experimental field conditions.

Project 2 "Chitosan bioactive films extracted from Agaricus bisporus fungus crops by-products for the protection of stored fruit - Act-NOS. The Act-NOS project aims at developing an edible film product to increase the shelf life of fruit. The specific objectives of the project are (1) recovery of chitin from Agaricus bisporus and conversion to chitosan; (2) obtaining chitosan-based films and hydrogels and bioactive monoaldehydes for bio-application; (3) expanding the concept of hydrogel and bioindustrial films to obtain complex systems with practical uses. which encapsulate new bioactive agents, aiming at their synergistic release.

Project 3 "Tribiotic products - probiotic, prebiotic, postbiotic - with multiple uses, obtained from by-products of vegetable industrialization - 3-4-Life". The 3-4-LIFE project aims to develop a bionanotechnology to produce tribiotics. The objectives of the 3-4-LIFE project are: (i) recovery of components from agro-food by-products, by extraction (enzymatic / intensified) and concentration, for nano-formulation and coacervation; (ii) building complex structures for the protection of lactic bacteria by coacervating nano-encapsulated prebiotics together with lactic bacteria; (iii) the development of the semisolid fermentation process, using the free lipid yeasts Y. and the lactic bacteria protected in complex structures.

Project 4 "New generation cosmetic products, obtained through the superior valorisation of active ingredients from some bio-economy sub-products - PRENDE". The PRENDE project aims to obtain new cosmetic products based on bioactive peptides made up of sub-products of the fish industry. The objectives are to develop new active ingredients from bio-economy sub-products, mainly fish sub-products, through patented technologies, for the manufacture of new generation cosmetic products; O2) development of new nanostructure technologies for bioactive compounds for effective release in skin tissues and O3) development of new products and verification of biological properties and activity, new experimental cell culture models with inflammation and using optimized methods of analyzing the ability of products to effectively restore cellular balance.

Project 5 "Conversion of surplus wool in keratinous composite biomaterials with medical applications - KERAMED" The KERAMED project aims at developing keratin biomaterials with medical applications. The specific objectives of the KERAMED project are: (i) the valorisation of highly keratin waste; (ii) improving the properties of keratinous biomaterials; (iii) obtaining dermal patches and verifying their biological activity.

Project 1, NeXT-US. 3 patents of OSIM, 1 patent EPO.

1. Procedeu de ob?inere a suspensiilor stabile de nanoparticule de seleniu ?i silice asociate, autori Florin Oancea, Diana Constantinescu-Aruxandei, Iuliana R?ut, Mariana Constantin, cerere de brevet OSIM a2018 00616/28.08.2018

2. Procedeu de ob?inere a peptidelor bioactive ?i a proteinelor amfifile din substrat epuizat de la cultivarea ciupercilor lignocelulozice, Florin Oancea, Diana Constantinescu-Aruxandei, Otilia B?rbieru, Lumini?a Dimitiru, Naomi Tritean, cerere de brevet OSIM a2018 001014/03.012.2018

3. Biostimulant pe baz? de oligozaharine sinergizate ?i procedeu de ob?inere F. Oancea, D. Constantinescu-Aruxandei, Otilia B?rbieru, Lumini?a Dimitiru, Naomi Tritean. OSIM a2018 001012/03.012.2018

4.  Process for preparing stable suspensions of associated selenium and silica nanoparticles F Florin Oancea, Diana Constantinescu-Aruxandei, Iuliana R?ut, Mariana Constantin, Cerere brevet EPO EP18191359.1/…….2018

Project 2, Act-NOS. 4 articles, 2 patents, 11 communications

Articles in specialized journals

1.Daniela Ailincai, Liliana Mititelu Tartau, Luminita Marin, “Drug delivery systems based on biocompatible imino-chitosan hydrogels for local anticancer therapy”, Drug Delivery, 2018, 25, 1080-1090. (IF= 3,095)

2.Anda Olaru, “Cinammyl-imine-chitosan hydrogels. Morphology control”, Acta Chemica Iasi, 2018, 26-2.(BDI)

3. Anda Mihaela Craciun, Liliana Mititelu Tartau, Mariana Pinteala, Luminita Marin, “Nitrosalicyl-imine-chitosan hydrogels based drug delivery systems for long term sustained release in local therapy”, Journal of Colloid and Interface Science – accepted.(IF = 5,091)

4. E. Georgescu, A. Oancea, F. Georgescu, A. Nicolescu, E. I. Oprita, L. Vladulescu, M.-C. Vladulescu, F. Oancea, S. Shova, C. Deleanu, “Schiff bases containing a furoxan moiety as potential nitric oxide donors in plant tissues”, Plos One, 2018, 13 (7), e0198121. DOI 10.1371/journal.pone.0198121. (IF = 2,776)

Conferences presented at international events

1. Luminita Marin, “Chitosan Hydrogelation with Monoaldehydes – A Synthetic Approach Towards Multifunctional Biomaterials”, 14th IUPAC International Conference on Novel Materials and their Synthesis (NMS-XIV), Guangzhou, China, 2018.

Oral communication at international events

1.Manuela Maria Iftime, Luminita Marin, “Chiral hydrogels based on chitosan and betulinic aldehyde”, 4th International Conference on Bio-based Polymers and Composites (BiPoCo), Balatonfüred, Ungaria, 2018.

2.Daniela Ailincai, “Biocompatible hydrogels based on the self-assembling of citril-imino-chitosan dynamer”, The 11th Edition of the Symposium with International Participation, New Trends And Strategies In The Chemistry Of Advanced Materials With Relevance In Biological Systems, Technique And Environmental Protection, Timisoara, Romania, 2018.

3. Daniela Ailincai, Mihai Mares, Andra-Cristina Bostanaru, Mariana Pinteala, Luminita Marin, “Biomaterials with strong antimicrobial properties based on dynamic iminochitosan derivatives”, First Balkan Conference of Medical Mycology and Mycotoxicology – Balkan Fungus, Timisoara, Romania, 2018.

4. Luminita Marin, “Chitosan hydrogelation with monoaldehydes: a synthetic approach towards multifunctional biomaterials”, 4th International Conference on Bio-based Polymers and Composites (BiPoCo), Balatonfüred, Ungaria, 2018.

Oral communications at national events

1.Manuela-Maria Iftime, Lumini?a Marin, “Chiral betulin-imine chitosan hydrogels”, A XXXV-a  Conferin?a National? de Chimie, C?lim?ne?ti-C?ciulata, 2018.

Posters at international events

1. Manuela-Maria Iftime, “Dynamic salicyl-imino-chitosan hydrogels based on reversible imine linkage”, 4th International Conference on Bio-based Polymers and Composites (BiPoCo), Balatonfüred, Ungaria, 2018.

2. Daniela Ailincai, “Biocompatible hydrogels based on imino-boronate motif as promising materials for the treatment of Candidisis”, 4th International Conference on Bio-based Polymers and Composites (BiPoCo), Balatonfüred, Ungaria, 2018.

3. Daniela Ailincai, “Biocompatible hydrogels based on citril-imino-chitosan dynamer: synthesis, characterization, applications”, 4th International Conference on Bio-based Polymers and Composites (BiPoCo), Balatonfüred, Ungaria, 2018.

4. Andrei Bejan, Anda Mihaela Olaru, Luminita Marin, “Novel luminescent hydrogels based on chitosan”, 4th International Conference on Bio-based Polymers and Composites (BiPoCo), Balatonfüred, Ungaria, 2018.

Posters at national events

1. Ana-Maria Macsim, Emilian Georgescu, Alina Nicolescu, Florentina Georgescu, Florin Oancea, Calin Deleanu, “Multinuclear NMR characterization of Schiff bases containing a furoxan moiety”, A XXXV-a Conferin?a National? de Chimie, C?lim?ne?ti-C?ciulata, 2018.

2. Gabriela-Liliana Ailiesei, Manuela Iftime, Alina Nicolescu, Luminita Marin, Calin Deleanu, “NMR evaluation of urea release from salicyl-imine-chitosan hydrogels” A XXXV-a Conferin?a National? de Chimie, C?lim?ne?ti-C?ciulata, 2018.

Patents

1. Film bioactiv glicodinameric pe baz? de chitosan ob?inut din ciuperci ?i procedeu de aplicare. Autori: Florin Oancea, Lumini?a Marin, Diana Constantinescu Aruxandei, Daniela Ailinc?i, Alina Nicolescu, Calin Deleanu., Cerere brevet OSIM a2018 001027/03.12.2018

2. Xerogel pe baz? de chitosan cu eliberare controlat? a exo-semnalelor inductori de oxid nitric. Autori: Lumini?a Marin, Florin Oancea, Daniela Ailinc?i, Diana Constantinescu Aruxandei, Manuela Iftime, Calin Deleanu. Cerere brevet OSIM a2018 001028/03.12.2018

 POM and SEM images of glycidameric hydrogels before (a, c) and after encapsulation (b, d). 

Project 3, 3-4Life. 2 patents of OSIM

1.Ingrediente microîncapsulate pe baz? de extracte antocianice din coji de vinete ?i peptide bioactive din zer cu func?ionalitate ridicat? pentru utiliz?ri în industria alimentar?, autori: Nina Nicoleta Condurache, Gabriela Elena Bahrim, Gabriela Râpeanu ?i Nicoleta St?nciuc, CBI no. 25042/05.10.2018.

2. Ingrediente func?ionale formulate pe baz? de extracte flavonoidice din coji de ceap? ?i peptide bioactive din zer cu func?ionalitate ridicat? pentru utiliz?ri în industria alimentar?, autori: Nina Nicoleta Condurache, Liliana Mihalcea, Gabriela Elena Bahrim, Gabriela Râpeanu ?i Nicoleta St?nciuc, CBI no. 30564/27.11.2018.

Project 4, PRENDE. 1 article accepted for publication, 2 OSIM patent applications, 3 communications

Tatia R, Z?laru C, ?ârcomnicu I, Moldovan L, Cr?ciunescu O, C?linescu I, 2019, Isolation and characterizaton of hederagenin from Hedera helix L. extract with antitumor activity, Revista de Chimie, (acceptat? pentru publicare) (IF 1,232).

Patents

1.Procedeu de ob?inere a unor peptide colagenice antioxidante din piele de pe?te de ap? dulce. Cerere de brevet nr. a 2018 00882 din 8.11.2018, Solicitan?i: INCDSB Bucure?ti, ICECHIM Bucure?ti. Autori: Cr?ciunescu Oana, Gaspar Pintiliescu Alexandra, Moldovan Lucia, Oancea Anca Olgu?a, Oancea Florin.

2.Compozi?ie fotoprotectoare din oligopeptide bioactive ?i procedeu de ob?inere. Solicitant: INCDSB Bucure?ti. Autori: Cr?ciunescu Oana, Gaspar Pintiliescu Alexandra, ?tefan Laura Mihaela, Oancea Anca Olgu?a. Cerere de brevet nr. a2018 00937 din 22.11.2018

Comunications

1.         Oancea A, Cr?ciunescu O, U?oiu E, M?noiu S, Coroiu V, Stanciuc A-M, Moldovan L, New trends in using nanostructures and nanoparticles in (bio)materials functionalization, Prezentare oral? OP4.4 la International Conference of RSBMB, 5-7 sept 2018, Bucure?ti, România, Book of Abstracts.

3.         Savin S, Toma A, Cr?ciunescu O, Oancea A, Extraction and biological activities of chitin/chitosan from by-products of Ganoderma lucidum culture, prezentat? ca poster P05 la Simpozionul Interna?ional PRIOCHEM, 10-12 octombrie 2018, edi?ia a XIV-a, Bucure?ti, România, Sec?iunea 2. Bioresources, Biotechnologies and Bioinfection, Volume of Summaries.

4.         Toma A, Cr?ciunescu O, Tatia R, Oancea A, Comparative study on methods of extracting pectin from by-products of vegetables and fruits, prezentata ca poster P06 la Simpozionul International PRIOCHEM, 10-12 octombrie 2018, edi?ia a XIV-a, Bucure?ti, România, Sec?iunea 2. Bioresources, Biotechnologies and Bioinfection, Volume of Summaries.

Project 5, KERAMED, 2 patent applications from OSIM

1. Metod? rapid? pentru ob?inerea lanolinei cu grad înalt de puritate, din lâna de oaie, pentru formul?ri terapeutice, Dana Copolovici , Diana Constantinescu – Aruxandei, Lucian Copolovici, Alina Negoi, Simona Gavrila?, C?t?lina Cristea, Cristi Mois?, Florin Oancea, Loredana Laz?r, Andreea S?rsan, Florentina-Daniela Munteanu, CBI a2018 01028/05.12.2018

2. Metod? ecologic? pentru degradarea proteinelor din lân? , Dana Copolovici , Diana Constantinescu – Aruxandei, Lucian Copolovici, Alina Negoi, Simona Gavrila?, C?t?lina Cristea, Cristi Mois?, Florin Oancea, Loredana Laz?r, Andreea S?rsan, Florentina-Daniela Munteanu, CBI a2018 01029/05.12.2018

For completing the human resources at the level of the consortium, 23 new researchers were employed: INCDCP-ICECHIM - 11 (9 initially foreseen); ICMPP - 2; UDJ - 3; INSB - 4; UAV - 3.

The project objectives for this stage have been achieved. At the level of the complex project, actions have been taken to achieve the proposed objectives. One of the objectives of the PRO-SPER Complex Project was the development of a long-lasting partnership. Due to budget restructuring, checks were no longer available to strengthen the partnership. Emphasis was put on joint participation in joint trans-national competitions (ERA.NET, EEA Grants, Eurostar / Eureka), at least 2 partners in the consortium, for scale-up technologies developed to TRL4 through component projects and / or capitalizing on newly acquired knowledge. At this stage, four international project proposals, three EEA grants and a M.ERA.NET project were submitted in partnership. Also, in order to achieve the objectives of developing a long-term partnership for the provision of scientific and technological services in the priority field of the circular bio-economy and for mutually beneficial collaboration with the economic agents, including for the joint exploitation of project results and the promotion of excellence and of intelligent specialization by coagulating niche domains at the intersection of common nano-bio-technology concerns, the development efforts of the inter-regional research infrastructure for trans-disciplinary research for emerging nanobiotechnologies - RoRIC - NeXT-BioNAN have been continued. Based on the 10PCCDI PRO-SPER project, RoRIC - NeXT-BioNAN is a National Consortium, set up under the Partnership Agreement, which aims to link the activities of research infrastructures developed through efforts at each partner institution to support the leap of Knowledge in Bioeconomy / Bio-nano-technologies.

At the level of Project 1 "Developing a new generation of plant biostimulants by the superior valorisation of exhausted substrate from Pleurotus lignocellulosic culture - NeXT-US", 4 Experimental Models and 4 Experimental Procedures were developed for the bioproduct development of a new generation of biostimulants for plants. Three OSIM patent applications and 1 EPO patent application, were filed. EPO Patent Application EP18191359.1 exploits the ability of expansine and cellulases fungi to release phytosilicate from straw and chaff from the Pleurotus exhausted substrate to form biosilica nanoparticles or, in the case of addition of selenium salts, to form synergistic biogenic silica nanocomposites structures and zerovalent selenium.

At project level 2 "Bioactive Agents Based on Chitosan Extract from Agaricus bisporus for the Protection of Fruit Deposited - Act-NOS" were 3 experimental models and 3 experimental procedures for the development of an edible film product intended to increase the shelf life of the fruit. Two ISI articles, one BDI article, and one ISI article have been accepted for publication. Two OSIM patent applications and 11 scientific communications were submitted.

Within project 3 "Tribiotic products - probiotic, prebiotic, postbiotic - with multiple uses, obtained from by-products from the industrialization of vegetables - 3-4-Life", 7 Experimental Models and 5 Experimental Procedures were developed for the development of a bionanotechnology for obtaining some tribiotic products. Two patents have been filed.

Within Project 4 "New Generation cosmeceutical products, obtained through the superior valorisation of the active ingredients from some bio-economy sub-products - PRENDE", 6 Experimental Models and 4 Experimental Procedures developed for obtaining new cosmeceutical products based on bioactive peptide made from fishery industry sub-products. Two patent applications were filed, an ISI scientific paper was accepted, and three scientific communications were submitted.

At project level 5 "Conversion of surplus wool in keratinous composite biomaterials with medical applications - KERAMED" were developed 5 experimental models and 5 experimental procedures elaborated for the development of keratin based biomaterials with medical applications. Two OSIM patent applications were filed.

Overall in component projects, were published 2 Articles in ISI journals and 1 article published in journals indexed in international databases. Two ISI articles were accepted. 11 National Patent Applications, 1 International Patent Application, have been field. There have been 14 conferences. 23 young people were employed, of which 21 are supported only by the project components of the 10PCCDI PRO-SPER complex project.

Abstract 2019

The project objectives for  this  stage  have  been  achieved,  both  at the  level  of the complex project and at the level of the component projects.  One  of the  objectives of the PRO-SPER Complex  Project was  to develop  a   long-term   partnership. This year the activity focus on  joint   participation  in  national project calls (e.g. Sectoral DCI,  Research  on  the fortification of    food products with  functional ingredients  obtained  from  wine   by-products, or  PN-III-P2-2.1-PT-2019, Transfer to economic operators - PTE)   and  trans-national  calls (ERA.NET, EAA Grants, Eurostar / Eureka),  by at least 2 partners in the consortium, to  scale-up technologies  developed  till  TRL4  level through  component  projects and/or  by using newly  acquired  knowledge.  In   this   year,  1 sectoral proposal  (all  partners), 2  proposals  for  international projects, M.ERA.NET  and  BlueBio, 2  EEA  grants and 2 PTE projects were submitted  in  partnership .  In    addition, in order to achieve the objective    of  developing  a   long-term partnership was fulfilled also by the development  of  the inter-regional trans-disciplinary  research   infrastructure for emerging   nanobiotechnology  - RoRIC - NeXT-BioNAN has continued. Established  on the basis of the 10PCCDI PRO-SPER project, RoRIC – NeXT-BioNAN  is  a  National    Consortium,   developed  through  focused  efforts at the level  of each  partner institution, to  support the knowledge gap filling in  the  field  of  Bioeconomy  / Bio-nano-technologies. Thanks to  the inclusion  of RoRIc NeXT BioNAN on the  National Roadmap,  PRO-SPER  partners were     able to submit POC projects  in  section F - 2017-2018 -  Research infrastructure   projects   for  public CD   institutions/universities     (e.g. SMIS 127521  Inter-regional  cross-disciplinary research infrastructure for emerging  nanobiotechnologies  or  SMIS 1280199 Research Center  in  sustainable bio-eco-economy)

At   project  level 1 "Development  of a  new  generation of plant  biostimulants  by  superior   exploitation of the depleted substrate  from the cultivation of Pleurotus-NeXT-US lignocellulosic mushrooms" were    tested 4  Experimental models  and  4  experimental  processes, for the  development of a bioproduct of  the new  generation of plant biostimulants.   3  OEM patent   applications and  one  PCT/WIPO application have been submitted. The patent  filed  with OSIM/PCT  refers to a biostimulant with a  balanced    priming action  of  the plant  defense response, based on  fungal chitosan  and silica nanoparticles    suspended  in a  new natural eutectic solvent. Two  ISI   articles were  sent  for  publication and  12  scientific communications  were  supported.

At project level  2 "Bioactive films based  on  chitosan extracted from the mushroom by-products    Agaricus bisporus for the protection  of  stored fruit - Act-NOS"  were  tested 3  experimental models  and  3  experimental processes, for the  development of an edible  film  product  intended to  increase the shelf life of the fruit, as  well as for  the exploitation of the properties of  chitosan-based glycosides.    4 ISI  articles were  published,  1  OSI patent  application  was  submitted and  16  scientific communications  were  supported.

In   Project  3 "Tri-biotic products  – probiotics,  prebiotics, postbiotics – with multiple   uses,  obtained from  by-products  from the industrialization  of vegetables – 3-4-Life" tested 7  Experimental models  and  5  Experimental procedures, for the development of a bionanotechnologies for  obtaining  tribiotic products.  2   patents  of  invention were  filed,  2 ISI articles   were  published,  3  ISI  articles  were  sent  for  publication and  12  scientific communications  were  supported.

In  Project  4  "New   generation   cosmeceutical products,   obtained  by  making superior  use of active ingredients  from  some bioeconomy  sub-products  - PRENDE",  6  Experimental models  and  4  experimental procedures  developed to  obtain  new  cosmeceutical  products based on bioactive peptides consisting of sub-products  of the fish industry were developed.  2    patent    applications were   filed,  1 ISI article was    published, sent for  publication  1 other  ISI  article and  8  scientific communications  were   supported.

At Project  5 level "Conversion of  excess  wool  into keratin composite  biomaterials  with  medical applications  – KERAMED" were  developed  5  Experimental models  and  5  experimental procedures developed for the development  of   keratin-based biomaterials     with  medical applications . 1 ISI article was  published   (in  collaboration  with  project 4 PRENDE),  an APPLICATION for an OSI patent was  filed  (in  collaboration  with  project  1),  2  ISI  articles were sent  for  publication and  12  scientific communications  were  supported.

Overall     component projects were  published  7  Articles  in  ISI-listed  journals   and  8 ISI articles  were  published.  8 applications  for the National Patent, 1  application for the International Patent have been filed.  60   attendances were made  at the  conferences.  Despite staff  fluctuations,  the  23  young  employees were    maintained,  21  of whom are supported  only  by  the component projects  of the  complex project 10PCCDI PRO-SPER.   These  young people  have  supported  over  30  participations  in  conferences  and are co-authors  in 6  patent  applications and  4  articles  published  /sent    for  publication.

The project objectives for  this  stage  have  been  achieved,  both  at the  level  of the complex project and at the level of the component projects.  One  of the  objectives of the PRO-SPER Complex  Project was  to develop  a   long-term   partnership. This year the activity focus on  joint   participation  in  national project calls (e.g. Sectoral DCI,  Research  on  the fortification of    food products with  functional ingredients  obtained  from  wine   by-products, or  PN-III-P2-2.1-PT-2019, Transfer to economic operators - PTE)   and  trans-national  calls (ERA.NET, EAA Grants, Eurostar / Eureka),  by at least 2 partners in the consortium, to  scale-up technologies  developed  till  TRL4  level through  component  projects and/or  by using newly  acquired  knowledge.  In   this   year,  1 sectoral proposal  (all  partners), 2  proposals  for  international projects, M.ERA.NET  and  BlueBio, 2  EEA  grants and 2 PTE projects were submitted  in  partnership .  In    addition, in order to achieve the objective    of  developing  a   long-term partnership was fulfilled also by the development  of  the inter-regional trans-disciplinary  research   infrastructure for emerging   nanobiotechnology  - RoRIC - NeXT-BioNAN has continued. Established  on the basis of the 10PCCDI PRO-SPER project, RoRIC – NeXT-BioNAN  is  a  National    Consortium,   developed  through  focused  efforts at the level  of each  partner institution, to  support the knowledge gap filling in  the  field  of  Bioeconomy  / Bio-nano-technologies. Thanks to  the inclusion  of RoRIc NeXT BioNAN on the  National Roadmap,  PRO-SPER  partners were     able to submit POC projects  in  section F - 2017-2018 -  Research infrastructure   projects   for  public CD   institutions/universities     (e.g. SMIS 127521  Inter-regional  cross-disciplinary research infrastructure for emerging  nanobiotechnologies  or  SMIS 1280199 Research Center  in  sustainable bio-eco-economy)

At   project  level 1 "Development  of a  new  generation of plant  biostimulants  by  superior   exploitation of the depleted substrate  from the cultivation of Pleurotus-NeXT-US lignocellulosic mushrooms" were    tested 4  Experimental models  and  4  experimental  processes, for the  development of a bioproduct of  the new  generation of plant biostimulants.   3  OEM patent   applications and  one  PCT/WIPO application have been submitted. The patent  filed  with OSIM/PCT  refers to a biostimulant with a  balanced    priming action  of  the plant  defense response, based on  fungal chitosan  and silica nanoparticles    suspended  in a  new natural eutectic solvent. Two  ISI   articles were  sent  for  publication and  12  scientific communications  were  supported.

At project level  2 "Bioactive films based  on  chitosan extracted from the mushroom by-products    Agaricus bisporus for the protection  of  stored fruit - Act-NOS"  were  tested 3  experimental models  and  3  experimental processes, for the  development of an edible  film  product  intended to  increase the shelf life of the fruit, as  well as for  the exploitation of the properties of  chitosan-based glycosides.    4 ISI  articles were  published,  1  OSI patent  application  was  submitted and  16  scientific communications  were  supported.

In   Project  3 "Tri-biotic products  – probiotics,  prebiotics, postbiotics – with multiple   uses,  obtained from  by-products  from the industrialization  of vegetables – 3-4-Life" tested 7  Experimental models  and  5  Experimental procedures, for the development of a bionanotechnologies for  obtaining  tribiotic products.  2   patents  of  invention were  filed,  2 ISI articles   were  published,  3  ISI  articles  were  sent  for  publication and  12  scientific communications  were  supported.

In  Project  4  "New   generation   cosmeceutical products,   obtained  by  making superior  use of active ingredients  from  some bioeconomy  sub-products  - PRENDE",  6  Experimental models  and  4  experimental procedures  developed to  obtain  new  cosmeceutical  products based on bioactive peptides consisting of sub-products  of the fish industry were developed.  2    patent    applications were   filed,  1 ISI article was    published, sent for  publication  1 other  ISI  article and  8  scientific communications  were   supported.

At Project  5 level "Conversion of  excess  wool  into keratin composite  biomaterials  with  medical applications  – KERAMED" were  developed  5  Experimental models  and  5  experimental procedures developed for the development  of   keratin-based biomaterials     with  medical applications . 1 ISI article was  published   (in  collaboration  with  project 4 PRENDE),  an APPLICATION for an OSI patent was  filed  (in  collaboration  with  project  1),  2  ISI  articles were sent  for  publication and  12  scientific communications  were  supported.

Overall     component projects were  published  7  Articles  in  ISI-listed  journals   and  8 ISI articles  were  published.  8 applications  for the National Patent, 1  application for the International Patent have been filed.  60   attendances were made  at the  conferences.  Despite staff  fluctuations,  the  23  young  employees were    maintained,  21  of whom are supported  only  by  the component projects  of the  complex project 10PCCDI PRO-SPER.   These  young people  have  supported  over  30  participations  in  conferences  and are co-authors  in 6  patent  applications and  4  articles  published  /sent    for  publication.

Scientific papers

1. Designing chitosan based eco-friendly multifunctional soil conditioner systems with urea controlled release and water retention; M-M. Iftime, G.L. Ailiesei, E. Ungureanu, L. Marin; Carbohydrate Polymers, 223, 115040 (2019). DOI: 10.1016/j.carbpol.2019.115040. (Factor de impact 6,044) ISSN: 0144-8617; Scor relative influenta:  2.726

2. Theoretical modelling of long time drug release from nitrosalicyl-imine-chitosan hydrogels through multifractal logistic type laws; A. M. Craciun, M. L. Barhalescu, M. Agop, L. Ochiuz; Computational and Mathematical Methods in Medicine, 2019, Article ID 4091464 (2019). DOI: 10.1155/2019/4091464. (Factor de impact 1,563.) 1748-670X 0.436

3. Nanoporous furfuryl-imine-chitosan fibers as a new pathway towards eco-materials for CO2 adsorption; L. Marin, B. Dragoi, N. Olaru, E. Perju, A. Coroaba, F. Doroftei, G. Scavia, S. Destri, S. Zappia, W. Porzio; European Polymer Journal, 120, 109214 (2019). DOI: 10.1016/j.eurpolymj.2019.109214. (Factor de impact 3.741) 0014-3057 2.308

4. Nitrosalicyl-imine-chitosan hydrogels based drug delivery systems for long term sustained release in local therapy; A. M. Craciun, L. Mititelu Tartau, M. Pinteala, L. Marin; Journal of Colloid and Interface Science 536, 196–207 (2019). DOI: 10.1016/j.jcis.2018.10.048. Factor de impact 6,361; 0021-9797 1.431.

5. Closing the Loop from  the begining– Polysaccharides and Oligosaccharides recovery during Biomass Biorefinery, D. Constantinescu-Aruxandei, Phillipe Michaud, Otilia B?rbieru, Florin Oancea, Applied Sciences.

6. Plant Biostimulants Effects on Tomatoes of Baker’s Yeast Vinasse and Chitosan Included in a Foliar Fertilizer, Stefan-Ovidiu Dima, Constantin Neam?u, Malina Desliu-Avram, Marius Ghiurea, Luiza Capra, Elena Radu, Rus?ndica Stoica, Victor-Alexandru Faraon, Valentin Zamfiropol-Cristea, Diana Constantinescu-Aruxandei, Florin Oancea, Agronomy, 2020,10(1), 133; https://doi.org/10.3390/agronomy10010133

7. Cristian Moisa, Andreea Lupitu, Georgeta Pop, Dorina Chambre, Lucian Copolovici, Gabriela Cioca, Simona Bungau, Dana Maria Copolovici, Variation of the Chemical Composition of Thymus vulgaris Essential Oils by Phenological Stages, Revista de chimie, 2019, 70, 633-637 (I.F. = 1.605).  REVISTA DE CHIMIE 0034-7752 0.183

8. Condurache, N.N., Aprodu, I., Cr?ciunescu, O., Tatia, R., Horincar, G., Barbu, V., Enachi, E., Râpeanu, G., Bahrim, G.E., Oancea, A., St?nciuc, N. 2019. Probing the Functionality of Bioactives from Eggplant Peel Extracts through Extraction and Microencapsulation in Different Polymers and Whey Protein Hydrolysates, Food and Bioprocess Technology, 12, 1316-1329, https://doi.org/10.1007/s11947-019-02302-1; Impact factor: 3.449 (2018) 1935-5130;   2.023

9. Milea, A.S., Aprodu, I., Vasile, A.M., Barbu, V., Râpeanu, G., Bahrim, G.E., St?nciuc, N. 2019. Widen the functionality of flavonoids from yellow onion skins through extraction and microencapsulation in whey proteins hydrolysates and different polymers. Journal of Food Engineering, 251, 29-35, https://doi.org/10.1016/j.jfoodeng.2019.02.003 ; Impact factor: 3.625 (2018); 2194-5764 0.334

10. Milea, A.S., Aprodu, I., Mihalcea, L., Bolea, C.A., Râpeanu, G., Bahrim, G.E., St?nciuc, N. Structural insights into the binding mechanisms of flavonoids supercritical fluids extracted from yellow onion skins and bovine <beta>-lactoglobulin peptides using fluorescence spectroscopy, ligand docking and molecular dynamics simulations, Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, under review, SAA-D-19-02001.

11. Condurache, N.N., Aprodu, I., Grigore-Gurgu, L., Petre, B.A., Râpeanu, G., Bahrim, G.E., St?nciuc, N. Evidences on anthocyanins binding to peptides derived from bovine lactoferrin: a fluorescence spectroscopy and molecular modeling approach, Food Chemistry, under review, FOODCHEM-D-19-06480

12. Constantin, O.E., Milea, A.S., Bolea, C., Mihalcea L., Enachi E., Copolovici, D. M., Copolovici, Munteanu, L.F., Bahrim, G.E., Rapeanu, G. Onion (Allium cepa L.) peel extracts characterization by conventional and modern methods, European Food Research and Technology, EFRT-19-1385.

13. Tatia R, Zalaru C, Tarcomnicu I, Moldovan L, Craciunescu O, Calinescu I, 2019, Isolation and characterizaton of hederagenin from Hedera helix L. extract with antitumor activity, Revista de Chimie, 70(4), 1157-1161 (IF 1,605) . 0034-7752 0.183

14. Iosageanu A, Oancea A, Ilie D, Anton ED, Craciunescu O, 2019, The effect of fish bioactive peptides on the wound healing process: an in vitro study on keratinocytes, Romanian Biotechnological Letters, (IF 0,404); 1224-5984 0.146

15. Simona Gavrila?, Andreea Sarsan, Cristi Mois?,  Dorina Chambre, Bianca Chereji, Lucian Copolovici, Dana Copolovici, Florin Oancea, Florentina-Daniela Munteanu, Valorisation of keratin-rich waste in the light of circular economy, Sustainability, (FI = 2,592) Sustainability Science 1862-4065 1.885

Communications

1. Natural deep eutectic solvents – preparation, characterisation and applications in green chemistry, Alina Negoi^1#, C?t?lina-Diana Cristea^1#, Alina Nicolescu², Sanda-Maria Doncea¹, Mariana C?lin, Ioana-Cristina Marina?¹, Eliza-Gabriela Mih?il?¹, Diana Constantinescu-Aruxandei^1*, Florin Oancea^1*. Innovative trans-sectoral technologies based on chemistry - NeXT-CHEM, 06/05/2019 - 07/05/2019, Bucuresti
2. Cascade processing and valorisation of ?urcana wool, Eliza-Gabriela Mih?il?^1#, Alina Elena Negoi^1#, C?t?lina-Diana Cristea^1#, Luiza Capr?¹, Sanda-Maria Doncea¹, Elena Radu¹, Carmen-Valentina Nicolae¹,  Diana Constantinescu-Aruxandei^1*, Florin Oancea^1*. Innovative trans-sectoral technologies based on chemistry - NeXT-CHEM, 06/05/2019 - 07/05/2019, Bucuresti
3. Bioactive peptides from agaricus bisporus mushroom, NaomiTritean^1#, Otilia-Gabriela B?rbieru^1#, Diana Constantinescu-Aruxandei^1*, Florin Oancea¹. Innovative trans-sectoral technologies based on chemistry - NeXT-CHEM, 06/05/2019 - 07/05/2019, Bucuresti
4. Never-dried bacterial nanocellulose for biomedical and cosmetic applications, Carmen-Valentina Nicolae^1#, C?t?lina-Diana Cristea^1#, ?tefan-Ovidiu Dima¹, Sanda-Maria Doncea¹, Bogdan Tric?¹, Cristian-Andi Nicolae¹, Diana Constantinescu-Aruxandei¹, Florin Oancea^1*. Innovative trans-sectoral technologies based on chemistry - NeXT-CHEM, 06/05/2019 - 07/05/2019, Bucurest
5. Enhancing the antioxidant activity of polyphenols and honey, Lumini?aDimitriu^1,2#, Ioana-CristinaMarina?^1#, Sanda-Maria Doncea¹, Andra Nichi?ean^2,3, Diana Constantinescu-Aruxandei^1*, Florin Oancea^1*. Innovative trans-sectoral technologies based on chemistry - NeXT-CHEM, 06/05/2019 - 07/05/2019, Bucharest,
6. Mushrooms as potential alternative source of chitin and chitosan, C?t?lina-Diana Cristea ^1#, Alina Negoi^1#, Carmen Nicolae^1#, Elvira Alexandrescu¹, Bogdan Tric?¹, Sanda-Maria Doncea¹, Diana Constantinescu-Aruxandei¹, Florin Oancea^1*. Innovative trans-sectoral technologies based on chemistry - NeXT-CHEM, 06/05/2019 - 07/05/2019, Bucuresti.
7. Encapsulation of an enhanced SCOBY consortium, Ioana-Alexandra Bala^1#, Patricia Riurean ^2#, Angela Moraru^3,2, Florentina Matei ³, C?t?lina-Diana Cristea, Lumini?a Dimitriu, Diana Constantinescu-Aruxandei¹, Florin Oancea^1,3*. Innovative trans-sectoral technologies based on chemistry - NeXT-CHEM, 06/05/2019 - 07/05/2019, Bucuresti
8. Chitosan imination with monoaldehydes – a synthetic approach towards multifunctional hydrogels; L. Marin; European Polymer Congress, Hersonissos Heraklion Crete, Grecia, 9-14 Iunie, 2019.
9. Chitosan hydrogelation with monoaldehydes: a new strategy to multifunctional biomaterials; L. Marin, D. Ailincai, M.M. Iftime, A.M. Craciun, A. Bejan; 9th International Conference of the Chemical Societies of the South-East European Countries, Targoviste, Romania, 8-11 Mai, 2019.
10. Iminoboronate chitosan hydrogels – promising materials for the treatment of candidiasis; D. Ailincai, L. Marin; 9th International Conference of the Chemical Societies of the South-East European Countries, Targoviste, Romania, 8-11 Mai, 2019.
11. Citryl-imine-PEG-ylated chitosan hydrogels – promising materials for bioapplications; D. Ailincai, L. Marin; European Polymer Congress, Hersonissos Heraklion Crete, Grecia, 9-14 Iunie, 2019.
12. Fabrication of 2-formylphenylboronic acid-chitosan nanofibers and their application in wound healing; A. Anisiei, M. Mares, A.-C. Bostanaru, L. Marin; 13th Students' Congress of SCTM, Skopje, Macedonia de Nord, 19-21 septembrie 2019.
13. PEGylated phenothiazine derivatives as water soluble precursors for biomaterials; S. Cibotaru, D. Belei, L. Marin; 13th Students' Congress of SCTM, Skopje, Macedonia de Nord, 19-21 septembrie 2019.
14. Chitosan based copolymers with enhanced solubility properties; B. I.  Andreica, D. Ailincai, L. Marin; 13th Students’ Congress of SCTM, Skopje, Macedonia, 19-21 Septembrie. 2019.
15. Synthesis and characterization of a novel chitosan derivative using trimethylene carbonate; B. I.  Andreica, D. Ailincai, L. Marin; 9th International Conference of the Chemical Societies of the South-East European Countries, Targoviste, Romania, 8-11 Mai. 2019.
16. Water soluble Phenothiazine derivatives; S. Cibotaru, D. Belei, L. Marin, 9th International Conference of the Chemical Societies of the South-East European Countries, Targoviste, 8-11 May 2019.
17. Interlaboratory quantitation assessment for NMR metabolomics; G. Ailiesei, C. Dorneanu, A.-M. Macsim, C. Stavarache, L. Tirnacop, A. Nicolescu, C. Deleanu; 9th International Conference of the Chemical Societies of the South-East European Countries „Chemistry a Nature Challenger”, Targoviste, Romania, 8-11 mai. 2019
18. Nitrosalicyl-imine-chitosan hydrogels as matrix for drug delivery systems in local therapy; A. M. Craciun, M. Pinteala, L. Marin; European Polymer Congress, EPF 2019, Heraklion, Grecia, 9-14 Iunie. 2019.
19. Hidrogelarea chitosanului cu monoaldehide – o strategie promitatoare pentru obtinerea de eco-materiale sustenabile; L. Marin; Zilele Academice Iesene, Progrese in stiinta compusilor organici si macromoleculari, Iasi, Romania, 2-4 Octombrie, 2019.
20. Hidrogeluri pe baza de chitosan si salicilaldehida-sisteme de eliberare controlata a ureei; M-M. Iftime, L. Marin, G.L. Ailiesei, A 27-a Sesiune de comunicari stiintifice „Progrese in stiinta compusilor organici si macromoleculari”, organizata in cadrul Zilelor Academice Iesene, Iasi, Romania, 2-4 octombrie.2019.
21. Iminoboronate-chitosan nanofibers for wound healing application; A. Anisiei, M. Mares, A.-C. Bost?naru, L. Marin; Sesiunea de comunic?ri ?tiin?ifice a studen?ilor, masteranzilor si doctoranzilor, Ia?i, Romania, 20-21 iunie 2019.
22. Novel chitosan derivatives with improved solubility properties; B. I.  Andreica, D. Ailincai, L. Marin; Sesiunea de comunic?ri ?tiin?ifice a studen?ilor, masteranzilor ?i doctoranzilor  "Chimia - frontier? deschis? spre cunoa?tere", edi?ia a X-a, Iasi, Romania, 20-21 iunie.  2019.
23. Water soluble phenothiazine derivatives; S. Cibotaru, D. Belei, E. Bîcu, L. Marin; Sesiunea de comunic?ri ?tiin?ifice a studen?ilor, masteranzilor ?i doctoranzilor. "Chimia - frontier? deschis? spre cunoa?tere", Ia?i, Romania, 20-21 iunie 2019.
24. St?nciuc, N., Bahrim, G.E., Râpeanu, G. 2019. 3-4-Life - A transition from Probiotics to Postbiotics as Novel Approach for Functional Ingredients and Foods. EuroAliment Symposium, Gala?i, 5-6 September.
25. St?nciuc, N. 2019. Tailoring the Functional Benefits of Bioactives from Food by-products: From “Gutter-to-Gold”. 2nd ICGEB Workshop “Modern Biotechnological Advances for Human Health”, Bucure?ti, 28th – 31st May.
26. St?nciuc, N. 2019. Bioeconomie circular?: Modalit??i de valorificare a subproduselor agroalimentare în ingrediente multifunc?ionale, Ingredients Shows 2019, Sinaia, România.
27. Bolea, C., Enachi, E., Grigore-Gurgu, L., St?nciuc, N., Bahrim, G. 2019. Extraction and characterization of keratin from sheep wool.  EuroAliment Symposium, Gala?i, 5-6 September.
28. Milea, S.A., Mihalcea L., Bahrim G.E., Vasile A.M., Râpeanu G., St?nciuc N. 2019. Ingrediente func?ionale formulate pe baz? de extracte flavonoidice din coji de ceap? galben? ?i peptide bioactive din zer pentru utiliz?ri în industria alimentar?. Inventica, Iasi, 26-28 June 2019,
29. Milea, S.A, Bahrim G.E., Râpeanu G., St?nciuc N., 2019. Onion (Allium cepa L.) skin extracts characterization by conventional and modern methods, Seventh Edition of the Scientific Conference of the Doctoral Schools of Dun?rea de Jos University, Gala?i, 13-14 June 2019, honorable mention.
30. Milea, ?.A., Bahrim, G.E., Râpeanu, G., St?nciuc, N. 2019. Ingrediente multifunc?ionale pe baz? de extracte flavonoidice din coji de ceap? galben? ?i bacterii lactice co-microîncapsulate ?i aplica?ii ale acestora. UGAL Invent, Gala?i, 16-18 octombrie – medalie de argint.
31. Milea, ?.A., Bahrim, G.E., Râpeanu, G., St?nciuc, N. 2019. Ingrediente multifunc?ionale pe baz? de extracte flavonoidice din coji de ceap? galben? ?i bacterii lactice co-microîncapsulate ?i aplica?ii ale acestora. INFOINVENT - EXPOZI?IA INTERNA?IONAL? SPECIALIZAT? - EDI?IA A XVI-A, 20 - 23 NOIEMBRIE 2019 - medalie de aur.
32. Condurache, N.N., Bahrim, G.E., St?nciuc, N., Râpeanu, G. 2019 – Bioactive compounds and antioxidant activity of extracts from eggplant peel using different extraction methods. Scientific Conference of Doctoral Schools the seventh edition, Gala?i, 13th-14th june - Second prize award and Prof. Constantin Moraru award.
33. Condurache, N.N., Bahrim, G.E., Râpeanu, G., St?nciuc, N. 2019 – Microencapsulated ingredients based on anthocyanins extract from eggplant peels and whey bioactive peptides with high functionality for use in food industry. The 23rd International Exhibition Of Inventics “Inventica”, Ia?i, 26-28 june.
34. Condurache, N.N., Bahrim, G.E., Râpeanu, G., St?nciuc, N. 2019 - Ingrediente naturale cu func?ionalitate multipl? pe baz? de extracte antocianice din coji de vinete ?i bacterii lactice co-microîncapsulate ?i aplica?ii ale acestora. UGAL Invent, Gala?i, 16-18 octombrie - Silver medal.
35. Condurache, N.N., Bahrim, G.E., Râpeanu, G., St?nciuc, N. 2019 - Ingrediente naturale cu func?ionalitate multipl? pe baz? de extracte antocianice din coji de vinete ?i bacterii lactice co-microîncapsulate ?i aplica?ii ale acestora. “INFOINVENT„ EXPOZI?IA INTERNA?IONAL? SPECIALIZAT? EDI?IA A XVI-A, Chi?in?u, Rep. Moldova, 20 - 23 noiembrie – Gold medal
36. Craciunescu O, Oancea A, Gaspar-Pintiliescu A, Ilie D, Anton ED, Iosageanu A, Oancea F, 2019, Isolation of antioxidant peptides from fish by-products, Book of Abstracts, p. 71, 2nd ICGEB Workshop “Modern Biotechnological Advances for Human Health” BAHH, 28-31 mai 2019, Bucuresti, Romania, ISBN: 978-973-0-29391-3 (poster presentation PP9)
37. Ilie D, Anton ED, Tatia R, Copolovici DM, Munteanu F, Copolovici L, Craciunescu O, 2019, Antioxidant and radioprotective activity of polyphenolic extracts of vegetable peels, 21st Romanian International Conference on Chemistry and Chemical Engineering (RICCCE), Constanta-Mamaia, Romania, 4-7 sept 2019 (poster presentation)
38. Gavrila? S, S?rsan AR, Dochia M, Copolovici L, Copolovici DM, Laz?r LN, Munteanu FD, 2019, Natural dyes extracted from food by-products with applications for natural fiber dyeing, 8thInternational Symposium Research and Education in Innovation Era, Arad, Romania, 23-25Mai 2019
39. Lupitu A, Mois? C, Rivi? A, Copolovici D, Copolovici L, 2019, Plant extracts for human health, 8th International Symposium Research and Education in Innovation Era, Arad, Romania, 23-25Mai 2019.
40. Mois? C, Lupitu A, Copolovici L, Copolovici D, Laz?r L, 2019, Essential oils and phenolic compounds, Bio-Sources and Their Applications, 2nd ICGEB Workshop Modern Biotechnological Advances for Human Health – BAHH, Bucuresti, Romania, 28-31 Mai 2019
41. Florentina-Daniela Munteanu, Simona Gavrila?, Lucian Copolovici, Andrea-Raluca S?rsan, Dana-Maria Copolovici, New And Traditional Methods Used for Keratin Extraction from Wool Fibers, 8^th International Symposium Research and Education in Innovation Era, May 23-25, 2019, Arad, Romania
42. Lucian Copolovici, Florentina Munteanu, Cristian Moisa, Dana Copolovici, Volatile Organic Compounds Emission - An Innovative Tool for Plants Stress and Food Characterization, 8^th International Symposium Research and Education in Innovation Era, May 23-25, 2019, Arad, Romania
43. Simona Gavrila?, Andrea-Raluca S?rsan, Mihaela Dochia, Lucian Copolovici, Dana-Maria Copolovici, Loredana-Nicoleta Laz?r, Florentina-Daniela Munteanu, Natural Dyes Extracted From Food By-Products With Applications for Natural Fiber Dyeing, 8^th International Symposium Research and Education in Innovation Era, May 23-25, 2019, Arad, Romania
44. Andreea Lupitu, Cristian Mois?, Adrian Rivi?, Dana Copolovici, Lucian Copolovici, Plant Extracts For Human Health, 8^th International Symposium Research and Education in Innovation Era, May 23-25, 2019, Arad, Romania
45. Cristian Mois?, Andreea Lupitu, Lucian Copolovici, Dana Copolovici, Loredana Laz?r, Essential Oils and Phenolic Compounds, Bio-Sources and Their Applications, 2^nd ICGEB Workshop “Modern Biotechnological Advances for Human Health – BAHH, May 28-31, 2019, Bucharest, Romania
46. Lucian Copolovici, Cristian Mois?, Andreea Lupitu, Dana Copolovici, The Implication of Plant Extracts in Human Health, 2^nd ICGEB Workshop “Modern Biotechnological Advances for Human Health – BAHH, May 28-31, 2019, Bucharest, Romania
47. Florentina-Daniela Munteanu, Lucian Copolovici, Simona Gavrila?, Andrea-Raluca S?rsan, Loredana-Nicoleta Laz?r, Cristian Moisa, Dorina Chambre, Dana-Maria Copolovici, New approaches for keratin extraction from sheep’s wool, 2^nd ICGEB Workshop “Modern Biotechnological Advances for Human Health – BAHH, May 28-31, 2019, Bucharest, Romania
48. Cristian Moisa, Lucian Copolovici, Andreea Lupitu, Dana Copolovici, Post-distillation waste materials – green sources of biological active compounds; the XII^th edition Environment & Progress, 15 November 2019, Cluj Napoca, Romania.
49. Cristian Moisa, Florentina Munteanu, Dana Copolovici, Florin Oancea, Lucian Copolovici, The protective role of mushroom extracts against plants cold stress, the V^th Edition of New Trends in Environmental and Materials Engineering, 23th – 25^th October 2019, Galati, Romania.
50. Cristian Moisa, Andreea I. Lupitu, Dana Maria Copolovici, Lucian Copolovici, Aromatic plants - bio-sources of essential oils and phenolic compounds, the 18th International ”Life Sciences for sustainable development” 26th– 28th September, 2019, Cluj-Napoca, Romania, (poster presentation).
51. Florentina-Daniela Munteanu, Simona Gavrilas, Raluca Sarsan, Loredana Lazar, Cristian Moisa, Dorina Chambre, Dana Maria Copolovici, Lucian Copolovici, Extraction method for keratin from sheep's wool, the 18th International ”Life Sciences for sustainable development” 26th– 28th September, 2019, Cluj-Napoca, Romania, (poster presentation).
52. Lucian Copolovici, Andreea Lupitu, Cristian Mois?, Dana Copolovici, Volatile organic compounds: from plants signalling to food fragrance, the 18th International ”Life Sciences for sustainable development” 26th– 28th September, 2019, Cluj-Napoca, Romania, (oral presentation).

Abstract 2020

The project objectives for this stage have been achieved, both at the level of the complex project and at the level of the component projects. One of the objectives of the complex project 10PCCDI PRO-SPER was the development of a  long-term partnership. It was emphasized that the consortium participated jointly in National and trans-National competitions, at least 2 partners in the consortium, to upscale the technologies developed till TRL4 level by component projects and/or to harness newly acquired knowledge.  At this stage,  the following  two projects have been contracted  to capitalize on the results and/or  competences developed in the frame of 10PCCDI PRO-SPER complex project: project RO-NO-2019-0540, Integrated use of the next generation plant biostimulants for an enhanced sustainability of field vegetable high residue farming systems  (ICECHIM+ICMPP), which harness the results of PC1 NeXT-US and PC2 component projects, Act-NOS and Project PN-III-P2-2.1-PTE-2019-0181  Biorefining of lateral flows resulting from the marketing of cyprinids  (INCDSB + ICECHIM), which up-scales the results of the PC4 PRENDE component project.

The consolidation of the component CDI institutions was also determined by the synergies resulting from the complementarity of the consortium members. This consortium brings together institutions that have developed significantly in the field of nanotechnology (ICMPP, UAV) and are interested in bio-applications, with institutions (UDJ, INSB), at which the biotechnology side has felt the need for a nanotechnology approach, for faster evolution. Coordination is carried out by INCDCP-ICECHIM, an institution in which the two directions, of nano and bio-technologies, have developed simultaneously. This complementarity ofthe consortium member facilitated the development of a long-term partnership for the provision of scientific and technological services in the priority area of the circular bioeconomy and for mutually beneficial collaboration with economic operators, including for the joint exploitation of project results. In addition, in order to achieve the objectives of developing a long-term partnership, providing scientific and technological services in the priority domain of the circular bioeconomy and for mutually beneficial collaboration with economic operators, including the joint exploitation of project results and the promotion of excellence and intelligent specialization in nano-bio-technology, the development of the inter-regional cross-disciplinary research infrastructure for emerging nanobiotechnology - RoRIC - NeXT-BioNAN has continued.

The joint CDI programme was  developed  and  assumed by all  partners since March 2020, 6 months before the initial end date of the complex project. The two main research directions of the CDI PRO-SPER Joint Programme are: (i) the sustainable intensification of bioresource production and (ii) the "intelligent" use of bioresources are geared towards countering the main weaknesses of the Romanian bioeconomy, low productivity, and low profitability.

At project level 1 "Development of a new generation of plant biostimulants by superior exploitation of the depleted substrate from the cultivation of Pleurotus-NeXT-US lignocellulosic mushrooms" the objectives for this stage have been achieved, achieving optimization of experimental models. 6 optimization experimental models and 4 optimized  experimental processes were optimized and exceeded – 6 optimization experimental models and 4 optimized experimental processes were optimized. 1 article was published and 4 articles were sent for publication. Two  technologies, 1 sequential extraction technology of the spent substrate and 1 dispersive conditioning technology of the plant biostimulant product  have been completed.

At project level 2 "Bioactive films based on chitosan extracted from the mushroom by-products Agaricus bisporus for the protection of stored fruit - Act-NOS" were optimized 3 experimental models  and 3 experimental processes. Two  technologies have been completed that integrate these models and processes, 1 NaDES extraction technology of fungal chitin and a chitosan-based glycodinamer technology for formation of bioactive films. 6 articles have been published  (compared to 2 planned)

In the frame of the project 3 "Tribiotic products – probiotics, prebiotics, postbiotics – with multiple uses, obtained from by-products from vegetable industrialization – 3-4-Life" were optimized 7 models and 5 related experimental processes, which were integrated into 4 technologies. There were 4 articles published  and sent for publication  4 other  articles.

The activities carried out under Project 4 have led to the achievement of the planned objectives. The result indicators were reached and exceeded. 6 experimental models and 4 experimental processes were optimized,  which were integrated into 2 technologies: 1 technology optimized and validated in the laboratory, being transferred to the economy by PT technology transfer project, 1 technology carried out, being validated in the laboratory. Two  articles were planned – sent for publication. 2 articles were published,  accepted 1 article, and sent for publication 2 articles.

At Project 5 level "Conversion of excess wool into keratin composite biomaterials with medical applications – KERAMED" were optimized at this stage 5 experimental models  and 5 experimental processes.  Two  articles were planned – sent for publication. 2  articles were accepted and sent for publication 5 articles."

Overall component projects were published/accepted upon publication 15 Articles in ISI-listed journals and 17 ISI articles were sent to the publication. 25 presentation and/or posters were done at various scientific  conferences. Despite the staff fluctuations, the 21 young employees were maintained and supported only by the component projects of the complex project 10PCCDI PRO-SPER. These young people have presented over 12 oral presentations at different conferencesa and are co-authors of articles published/sent for publication.

Publications

1. Nanocellulose Hybrids with Metal Oxides Nanoparticles for Biomedical Applications, 2020,  Madalina Oprea,  Denis Mihaela Panaitescu, Molecules, Molecules 2020, 25, 4045; doi:10.3390/molecules25184045

2. Bionanotechnologies potential to optimize Romanian circular bioeconomy, 2020, Sustainability/ Diana Constantinescu-Aruxandei,  Florin Oancea/în evaluare

3. Structural analysis of cabbage leaves treated with an plant biostimulant, Agronomy, 2020,  Stefan-Ovidiu Dima, Marius Ghiurea, Claudia-Mihaela Ninciuleanu, Constantin Neamtu, Victor-Alexandru Faraon, Diana Constantinescu-Aruxandei, Florin Oancea/în evaluare

4. Biostimulant seed coating based on silica nanoparticles, Agronomy, 2020,  Naomi Tritean, Bogdan Tric?, ?tefan-Ovidiu Dima, Victor Faraon, Elvira Alexandrescu, Paolina Lukova, Cédric Delattre, Diana Constantinescu-Aruxandei, Florin Oancea /în evaluare

5. Polysaccharides seed coating – multifunctional tools for a sustainable agriculture, Polymers, 2020, Otilia Gabriela B?rbieru, Bogdan Tric?, Naomi Tritean, Paolina Lukova, Cédric Delattre, Diana Constantinescu-Aruxandei, Florin Oancea / în evaluare

6. Electrospinning of chitosan based nanofibers: from design to applications, Reviews in Chemical Engineering, 2020, Alexandru Anisiei, Florin Oancea, Luminita Marin / în evalaure

7. Manuela-Maria Iftime, Liliana Mititelu Tartau, Luminita Marin, New formulations based on salicyl-imine-chitosan hydrogels for prolonged drug release, International Journal of Biological Macromolecules 160, 2020, 398-408, https://doi.org/10.1016/j.ijbiomac.2020.05.207

8. Daniela Ailincai, Liliana Mititelu-Tartau, Luminita Marin, Citryl-imine-PEG-ylated chitosan hydrogels – Promising materials for drug delivery applications, International Journal of Biological Macromolecules, 162, 2020, 1323-1337, https://doi.org/10.1016/j.ijbiomac.2020.06.218

9. Manuela Maria Iftime, Stefan Andrei Irimiciuc, Maricel Agop, Marian Angheloiu, Lacramioara Ochiuz, Decebal Vasincu, A Theoretical Multifractal Model for Assessing Urea Release from Chitosan Based Formulations, Polymers, 12, 2020, 1264; doi:10.3390/polym12061264.

10. Manuela Maria Iftime, Marian Angheloiu, Ultrasonication - A Potential Method Toward Chitosan Hydrogels, Materiale Plastice, 57, 2020, 67-77; https://doi.org/10.37358/MP.20.2.5352

11. Bianca-Iustina Andreica, Xinjian Cheng, Luminita Marin; Quaternary ammonium salts of chitosan. A critical overview on the synthesis and properties generated by quaternization, European Polymer Journal, 139, 2020, 110016; https://doi.org/10.1016/j.eurpolymj.2020.110016

12. Daniela Ailincai, Alexandra Maria Doroban?u , Bogdan Dima, ?tefan Andrei Irimiciuc, Cristian Lupa?cu, Maricel Agop, Orzan Olguta, Poly(vinyl alcohol boric acid)-Diclofenac Sodium Salt Drug Delivery Systems: Experimental and Theoretical Studies, Hindawi Journal of Immunology Research Volume, 2020, 3124304, 2020; https://doi.org/10.1155/2020/3124304

13. Condurache, Nina Nicoleta, Aprodu, Iuliana, Grigore-Gurgu, Leontina, Petre, Br?ndu?a Alina, Enache, Elena, Râpeanu, Gabriela, Bahrim, Gabriela Elena, St?nciuc, Nicoleta. (2020). Fluorescence spectroscopy and molecular modeling of anthocyanins binding to bovine lactoferrin peptides. Food Chemistry, 318, 126508, https://doi.org/10.1016/j.foodchem.2020.126508, Factor de impact 6,306.

14. St?nciuc, Nicoleta, Râpeanu, Gabriela, Bahrim, Gabriela Elena, Aprodu, Iuliana. (2020). The Interaction of Bovine β-Lactoglobulin with Caffeic Acid: From Binding Mechanisms to Functional Complexes. Biomolecules, 10, 1096, doi:10.3390/biom10081096, Factor de impact 4,082.

15. Cotârlet, Mihaela, St?nciuc, Nicoleta, Bahrim, Gabriela Elena. (2020). Yarrowia lipolytica and Lactobacillus paracasei Solid State Fermentation as a Valuable Biotechnological Tool for the Pork Lard and Okara’s Biotransformation. Microorganisms, 8, 1098, doi:10.3390/microorganisms8081098, Factor de impact 4,152.

16. Milea, ?tefania Adelina, Mihaela Aida Vasile, Oana Cr?ciunescu, Ana-Maria Prelipcean, Gabriela Elena Bahrim, Gabriela Râpeanu, Anca Oancea, Nicoleta St?nciuc (2020). Co-microencapsulation of flavonoids from yellow onion skins and lactic acid bacteria lead to a multifunctional ingredient for nutraceutical and pharmaceutics applications. Pharmaceutics, 12, 1053; doi:10.3390/pharmaceutics12111053. Factor de impact 4,421.

17. Milea, ?tefania Adelina, Aprodu, Iuliana, Enachi, Elena, Barbu, Vasilica, Râpeanu, Gabriela, Bahrim, Gabriela Elena, St?nciuc, Nicoleta.     β-lactoglobulin peptides enhanced the health-benefits of flavonoids from yellow onion skins extract through microencapsulation. Cyta-Journal of Food. Acceptat pentru publicare, Factor de impact 1,653.

18. Milea, ?tefania Adelina, Aprodu, Iuliana, Mihalcea, Liliana, Enachi, Elena, Bolea, Carmen Alina*, Râpeanu, Gabriela, Bahrim Gabriela Elena, St?nciuc, Nicoleta. Bovine β-lactoglobulin peptides as novel carries for flavonoids extracted with supercritical fluids from yellow onion skins. Journal of Food Science. Acceptat pentru publicare. Factor de impact 2,478.

19. Condurache, Nina Nicoleta, Enachi, Elena, Nistor, Oana, Bahrim, Gabriela Elena, St?nciuc, Nicoleta, Râpeanu Gabriela (2020). Effects of parameters variation on anthocyanins recovery from eggplant peels by using conventional and modern extraction methods, Foods, Submis, Factor de impact 4,091.

20. Oana Emilia Constantin, Milea, ?tefania Adelina, Bolea Carmen Alina, Mihalcea, Liliana, Enachi, Elena, Copolovici, Dana Maria, Copolovici, Lucian, Munteanu Florentina, Bahrim, Gabriela Elena, Râpeanu, Gabriela. Profiles of onion (Allium cepa L.) peel extracts studied for antioxidant compounds determination. Scientific Reports, Submis, Factor de impact 4,12.

21. Savin S, Craciunescu O, Oancea A, Ilie D, Ciucan T, Antohi LS, Toma A, Nicolescu A, Deleanu C, Oancea F, 2020, Antioxidant, cytotoxic and antimicrobial activity of chitosan preparations extracted from Ganoderma lucidum mushroom, Chemistry & Biodiversity, 17, e2000175. doi: 10.1002/cbdv.202000175

22. Grigore-Gurgu L, Craciunescu O, Aprodu I, Bolea CA, Iosageanu A, Petre BA, Bahrim GE, Oancea A, Stanciuc N, 2020, Tailoring the health-promoting potential of protein hydrolysate derived from fish wastes and flavonoids from yellow onion skins: from binding mechanisms to microencapsulated functional ingredients, Biomolecules, 10, 1416, doi: 10.3390/biom10101416

23. Iosageanu A, Oancea A, Ilie D, Anton ED, Craciunescu O, 2020, The effect of fish bone bioactive peptides on the wound healing process: an in vitro study on keratinocytes, Romanian Biotechnological Letters (acceptat pentru publicare)

24. Ilie D, Iosageanu A, Craciunescu O, Oancea A, Anton ED, Sanda C, Oancea F, 2020, Antioxidant activity and effect on fibroblast proliferation and migration of collagen hydrolysates from silver carp (Hypophthalmichthys molitrix) skin, Food Bioscience, (in evaluare)

25. Angela Moraru, Stefan-Ovidiu Dima, Elena-Iulia Oprita, Anca Oancea, Sanda-Maria Doncea, Raluca Somoghi, Ana-Maria Stanciuc, Lucia Moldovan, Cristian-Andi Nicolae, Diana Constantinescu-Aruxandei, Florin Oancea, Bioactive thermo-responsive hydrogels based on never-dried bacterial nanocellulose, Pharmaceutics, IF-4,421 / în evaluare.

26. Chemical composition, antioxidant capacity and thermal behavior of Satureja hortensis essential oil, 2020, Scientific Reports, Dorina Rodica Chambre, Cristian Moisa, Andreea Lupitu, Lucian Copolovici, Georgeta Pop, Dana-Maria Copolovici. Acceptat

27. The influence of different vacuum impregnation solutions on the colour of fresh-cut apple slices, 2020, Scientific Study & Research Chemistry & Chemical Engineering, Biotechnology, Food Industry, Simona Gavrila?, Claudiu-?tefan Ursachi, Mihaela Dochia, Simona Per?a-Cri?an, Florentina-Daniela Munteanu, Acceptat

28. Green synthesis, characterization, and antibacterial properties of silver nanoparticles obtained by using diverse varieties of Cannabis sativa leaf extracts, 2020, Antioxidants, Adriana Cecilia Csakvari, Dana G. Radu, Leonard M. Olariu, Andreea I. Lupitu, Cristian Moisa, Anca Ofelia Panda, Georgeta Pop, Dorina Chambre, Lucian Copolovici, Dana Maria Copolovici. In evaluare

29. The seasonality impact of the BTEX pollution on the atmosphere of Arad City, Romania, 2020, Air Quality, Atmosphere and Health, Lucian Copolovici, Gabriela Cioca, Corina Popitanu, Dennis Iosif, Florentina Munteanu, and Dana Maria Copolovici, In evaluare

30. Camelina sativa methanolic and ethanolic extracts potential in alleviating oxidative stress, memory deficits and affective impairments in stress exposure-based irritable bowel syndrome mice models, 2020, Oxidative Medicine and Cellular Longevity, Roxana O. Cojocariu, Ioana-Miruna Balmus, Radu Lefter, Luminita Hritcu, Daniela C. Ababei, Alin Ciobica, Simona Copaci, Silvia E.L. Mot, Lucian Copolovici, Dana M. Copolovici, Stefana Jurcoane, In evaluare

31. Simona Gavrila?, Andreea Sarsan, Mihaela Dochia, Cristi Mois?, Andreea Lupitu, Lucian Copolovici, Dana Copolovici, Florin Oancea, Florentina- Daniela Munteanu, Natural pigments from vegetable waste for dyeing of wool fibre, Dyes and Pigments In evaluare

32. Simona Gavrila?, Andreea S?rsan*, Cristi Mois?*, Dorina Chambre, Bianca Chereji*, Lucian Copolovici, Dana Copolovici, Florin Oancea, Florentina-Daniela Munteanu, Valorisation of keratin-rich waste in the light of circular economy, Sustainability, In evaluare

Sessions

1. Synthesis and characterization of nano-biosilica, Naomi Tritean, Bogdan Tric?, ?tefan-Ovidiu Dima, Diana Constantinescu-Aruxandei, Florin Oancea, Innovative trans-sectoral technologies based on chemistry – NeXT-CHEM, Bucuresti, 5-6 iunie 2020

2. Utilization of laccases in the presence of deep eutectic solvents for degradation of lignocellulosic biomass, Preda Daniel, Alina Elena Negoi, Ioana-Cristina Marina?, Diana Constantinescu-Aruxandei, Florin Oancea, Adina R?ducan, Innovative trans-sectoral technologies based on chemistry – NeXT-CHEM, Bucuresti, 5-6 iunie 2020

3. Ecofriendly extraction of polysaccharides from brewer’s spent grain with deep eutectic solvents, Alina Negoi, Carmen Valentina Nicolae, Ioana-Cristina Marina?, Marius Ghiurea, Alina Nicolescu, Sanda-Maria Doncea, Diana Constantinescu-Aruxandei, Florin Oancea, Innovative trans-sectoral technologies based on chemistry – NeXT-CHEM, Bucuresti, 5-6 iunie 2020

4. Optimization of Laccase Extraction from Spent Pleurotus Substrate, Daniel Preda, M?lina De?liu-Avram, Bogdan Tric?, Florin Oancea, Diana Constantinescu-Aruxandei, Priorities of chemistry for a sustainable development” PRIOCHEM - XVITH Edition, Bucureti, 28-30 octombrie 2020

5. Manuela Maria Iftime, Luminita Marin, New urea delivery systems based on salicyl-imine-chitosan hydrogels, 5^th International Conference on Chemical Engineering Romania (ICCE 2020), Ia?i, October 28 – 30, 2020.

6. Andrei Bejan, Florica Doroftei, Xinjian Cheng, Luminita Marin - Phenothiazine-chitosan based materials for mercury removal and fast naked eye detection - The 1^st International Electronic Conference on “Green” Polymer Materials 2020 (CGPM 2020), November 5–25, 2020, online at: https://cgpm2020.sciforum.net/.

7. Bianca-Iustina Andreica, Daniela Ailincai, Luminita Marin, Chitosan copolymers with improved solubility by Ring-Opening Polymerization technique, 5^th International Conference on Chemical Engineering Romania (ICCE 2020), Ia?i, October 28 – 30, 2020.

8. Daniela Ailinc?i, Luminita Marin, Iminoboronate chitosan hydrogels – a promising tool in the treatment of Candida infections, 5^th International Conference on Chemical Engineering Romania (ICCE 2020), Ia?i, October 28 – 30, 2020.

9. Alexandru Anisiei, Irina Rosca, Luminita Marin, Functionalized chitosan nanofibers with enhanced antimicrobial activity for burn wound healing applications, The 1^st International Electronic Conference on “Green” Polymer Materials 2020 (CGPM 2020), 5–25 November, 2020, online at: https://cgpm2020.sciforum.net/.

10. Sandu Cibotariu, Andreea-Isabela Sandu, Dalila Belei, Luminita Marin, Water soluble PEGylated phenothiazines. Synthesis, characterization and antitumor properties, The 1^st International Electronic Conference on “Green” Polymer Materials 2020 (CGPM 2020), 5–25 November, 2020, online at: https://cgpm2020.sciforum.net/.

11. Influence of parameters on different extraction methods of anthocyanins from eggplant peels. Nina Nicoleta Condurache, Elena Enachi, Gabriela Elena Bahrim, Nicoleta St?nciuc, Gabriela Râpeanu/8th edition of Scientific Conference of Doctoral Schools SCDS-UDJG, “Dunarea de Jos” University of Galati, 18-19 of June 2020.

12. Studies on binding mechanism between phenolic compounds from eggplant peels and lactoferrin. Nina Nicoleta Condurache, Gabriela Rapeanu, Iuliana Aprodu, Gabriela-Elena Bahrim, Nicoleta Stanciuc/8th edition of Scientific Conference of Doctoral Schools SCDS-UDJG, “Dunarea de Jos” University of Galati, 18-19 of June 2020.

13. Boosting the health-benefits of flavonoids from yellow onion skins and β-lactoglobulin peptides microencapsulation, Adelina ?tefania-Milea, Iuliana Aprodu, Gabriela Râpeanu, Gabriela Elena Bahrim, Nicoleta St?nciuc/8th edition of Scientific Conference of Doctoral Schools SCDS-UDJG, “Dunarea de Jos” University of Galati, 18-19 of June 2020.

14. Evidences of binding mechanism between flavonoids supercritical fluids extracted from yellow onion skins and bovine β-lactoglobulin peptides, Adelina ?tefania-Milea, Iuliana Aprodu, Gabriela Râpeanu, Gabriela Elena Bahrim, Nicoleta St?nciuc/8th edition of Scientific Conference of Doctoral Schools SCDS-UDJG, “Dunarea de Jos” University of Galati, 18-19 of June 2020.

15. Ingrediente naturale cu func?ionalitate multipl? pe baz? de extracte antocianice din coji de vinete ?i bacterii lactice co-microîncapsulate ?i aplica?ii ale acestora. Nina-Nicoleta Condurache, Gabriela-Elena Bahrim, Gabriela Râpeanu, Nicoleta St?nciuc/ INGREDIENTS SHOW BUCURE?TI 5-9 octombrie 2020

16. Iosageanu A, Ilie D, Sanda C, Craciunescu O, Oancea F, 2020, Protective activity of fish peptide fraction in optimized model of UV-B irradiated mouse fibroblasts, Simpozionul International PRIOCHEM XVI, Bucuresti, 28-30 oct 2020, la Sectiunea 2. Bioresurse si Biotehnologii.

17. International Conference "Agriculture for Life, Life for Agriculture", interna?ional?, Chemical Analyses And Potential Applications Of Plant Extracts., 2020 Silvia Elena Lavinia Mo? , Andreea Lupitu , Cristian Moisa , Daniela Mo? , Adina Maria Bodescu , Dorina Chambre , Smeurica Schitea , Lucian Copolovici , Dana M. Copolovici., June 4 - 6, 2020, Bucharest, Romania, ISSN 2343-9653 (PRINT), ISSN-L 2343-9653 poster presentation

18. International Conference "Agriculture for Life, Life for Agriculture", interna?ional?, Fish Protein Hydrolysates: Production And Applications, 2020, Dana Copolovici , Carmen Popa , Andreea Lupitu , Marilena Maereanu , Dorina Chambre , Cristian Moisa , Lucian Copolovici, June 4 - 6, 2020, Bucharest, Romania, ISSN 2343-9653 (PRINT), ISSN-L 2343-9653, oral presentation

19. International Conference "Agriculture for Life, Life for Agriculture", , interna?ional?, The Influence Of Paracetamol Metabolites To Plants Development, 2020,  Monica Taschina, Dana Copolovici, Andreea Lupitu, Cristian Moisa, Lucian Copolovici, June 4 - 6, 2020, Bucharest, Romania, ISSN 2343-9653 (PRINT), ISSN-L 2343-9653, poster presentation

20. International Conference "Agriculture for Life, Life for Agriculture", interna?ional?, Volatile Organic Compounds Emission from Plants Under Biotic Stress,2020, Lucian Copolovici, Andreea Lupitu, Cristian Moisa, Dana Copolovici, , June 4 - 6, 2020, Bucharest, Romania, ISSN 2343-9653 (PRINT), ISSN-L 2343-9653, oral presentation

21. The 19th International Conference ”Life Sciences For Sustainable Development, interna?ional?, Green Synthesis, Chemical And Biological Properties Of Silver Nanoparticles From Cannabis Sativa Leaf Extracts, 2020,  Dana Maria Copolovici, Adriana Csakvari, Dana G. Radu, Cristian Moisa, Georgeta Pop, Dorina Chambre, Andreea I. Lupitu, Lucian Copolovici,” 24 Th - 25 Th September 2020, Cluj-Napoca, Romania, Book Of Abstracts: ISSN: 2392-6937, ISSN-L: 2392-6937, oral presentation

22. The 19th International Conference ”Life Sciences For Sustainable Development, interna?ional?, The Influence Of Biotics Stress On Plants Metabolites, 2020, Lucian Copolovici1,2*, Corina Popitanu3, Cristian Moisa2, Andreea I. Lupitu2, Dana M. Copolovici, 24 Th - 25 Th September 2020, Cluj-Napoca, Romania, Book Of Abstracts: ISSN: 2392-6937, ISSN-L: 2392-6937, Oral Presentation

23. The 19th International Conference ”Life Sciences For Sustainable Development, interna?ional?, The Effect Of Drought Stress On The Photosynthetic Parameters Of Ocimum Basilicum, 2020,  L., Andreea I. Lupitu1,2*, Cristian Moisa1, Dana Maria Copolovici1 And Lucian Copolovici1, 24 Th - 25 Th September 2020, Cluj-Napoca, Romania, Book Of Abstracts: ISSN: 2392-6937, ISSN-L: 2392-6937, oral presentation

24. The 19th International Conference ”Life Sciences For Sustainable Development, interna?ional? Improving The Sensory And Chemical Characteristics Of Fruit Spirits By Macerating Dehydrated Fruits, 2020,  Maria Chifor1, Andreea Lupitu1, Cristian Moisa1, Dana Copolovici1, Sergiu Palcu1, Claudia Mure?an1, Lucian Copolovici 24 Th - 25 Th September 2020, Cluj-Napoca, Romania, Book Of Abstracts: ISSN: 2392-6937, ISSN-L: 2392-6937, oral presentation

25. The 19th International Conference ”Life Sciences For Sustainable Development, interna?ional? The Effect Of Light Intensity On The Accumulation Of Secondary Metabolites For Several Medicinal And Aromatic Plants 2020,  Cristian Moisa2*, Andreea I. Lupitu2, Lucian Copolovici1,2, Dana M. Copolovici, 24 Th - 25 Th September 2020, Cluj-Napoca, Romania, Book Of Abstracts: ISSN: 2392-6937, ISSN-L: 2392-6937, oral presentation

The project objectives for this stage have been achieved, both at the level of the complex project and the level of the component projects. One of the objectives of the Complex Project 10PCCDI PRO-SPER was the development of a  long-term partnership. It was emphasized that the consortium member jointly participated in National and trans-National / International competitions, at least two partners in the consortium, to raise the technologies developed up to TRL4 level by component projects and/or to harness newly acquired knowledge. The joint CDI programme, linked to the partners' institutional development plan, was complemented by research directions corresponding to the requirements of the European Green Deal, which uses side streams from the bioeconomy to reduce the carbon footprint of transportation and increase the production of carbon-negative biofuels. The integration of these directions on the use of side streams from the bioeconomy for CO₂ capture and the production of carbon-negative biofuels was the basis for the participation of project partners 10 PCCDI PRO-SPER in COST CA20127 - Waste biorefinery technologies for accelerating sustainable energy processes.

To achieve the objectives of developing a long-term partnership for the provision of scientific and technological services in the priority area of the circular bioeconomy and the beneficial collaboration with economic operators, including for the joint exploitation of project results and the promotion of excellence and smart specialization in the nano-bio-technology domain, the development of the Inter-Regional C Research Infrastructure for Emerging Bionanotechnologies - RoRIC - NeXT-BioNAN has continued. The need for the development of RoRIC – NeXT-BioNAN starts from the need to intensify research and innovation activities in nano-biotechnology. One way to step up the research and innovation activities envisaged is high-throughput experimentation technology  (HTT).

HTT is a generic name associated with a range of tools and techniques involving programmed experiments, simultaneous variation of several experimental parameters, and/or the parallel (bio)tests needed to optimize new technological solutions /new products. The value of this approach is determined by: (i) a better understanding of the determining parameters for the processing (bio)resources / (bio)intermediaries and for the rise to scale; (ii) rapid discovery of new and new (bio)materials with the characteristics necessary for specific applications; (iii)new scientific approaches, challenging to achieve with conventional equipment and processes.  The proposed RoRIC Next-BioNAN infrastructure has multiple roles: (i) achieving the leap of knowledge in the field of excellence and uniqueness of emerging nano-biotechnology and participating as an active actor in international partnerships, (ii) the training of new researchers, and (iii) the provision of CDI services to economic operators.  These multiple roles lead to a combined access policy, i.e., 'excellence-based access' for academia and young researchers and 'market-oriented, tax-based access' for economic operators/enterprises.

At project level 1, "Development of a new generation of plant biostimulants by making superior use of the exhausted substrate from the cultivation of Pleurotus-NeXT-US lignocellulosic mushrooms," the objectives for this stage have been achieved, achieving the finalization of the optimization of the experimental models. The result indicators were reached and exceeded – the optimization of 6 experimental models and four experimental processes was completed. 1  article  (Polymers,IF=4.329) was sent for publication, with the planned indicator. Two technologies, one for the sequential extraction of the spent mushroom substrate and one technology of dispersive formulation of the plant biostimulant, have been completed.

At project level 2, "Bioactive films based on chitosan extracted from the mushroom by-products Agaricus bisporus for the protection of stored fruit - Act-NOS" was completed the optimization of 3 experimental models and 3 experimental procedures.  Three technologies integrate these models and procedures, a NaDES extraction technology of macromycetes chitin and a chitosan-based glycodinamers technology for bioactive films, a technology for the use of glycodinameric hydrogels for other applications have been completed. 1  article has been published as planned.

In project 3, "Tribiotic products – probiotics, prebiotics, postbiotics – with multiple uses, obtained from by-products from vegetable industrialization – 3-4-Life" were optimized 7 models and 5 related experimental processes, which were integrated into 4 technologies. 2 articles were published and  sent for publication 2 articles–indicator, 1 article.

The activities carried out under Project 4 have led to the achievement of the planned objectives. The result indicators were reached and exceeded. 6 experimental models and 4 experimental processes were optimized,  which were integrated into 2 technologies:1 technology optimized and validated in the laboratory, transferred to the economy by PT technology transfer project, 1 technology carried out, and validated in the laboratory. 1 articles sent for publication were planned.  2  articles were published and sent for publication 1 article.

At Project 5 level "Conversion of excess wool into keratin composite biomaterials with medical applications – KERAMED" were optimized at this stage 5 experimental models and 5 experimental processes.  It was planned 1 article – sent for publication. It was published 1  article.

Overall component projects were published 6 Articles in ISI-listed journals and 4 other ISI articles were sent for publication. 6 attendances were made at the conferences. Despite staff fluctuations, the 21 newly created posts were maintained through the complex project 10PCCDI PRO-SPER. These young people presented 5 plenary conferences during this period and are co-authors of articles published/sent for publication.

Publications

•         Iosageanu A, Ilie D, Craciunescu O, Seciu-Grama AM, Oancea A, Zarnescu O, Moraru I, Oancea F, 2021, Effect of fish bone bioactive peptides on oxidative, inflammatory and pigmentation processes triggered by UVB irradiation in skin cells, Molecules, 26, 2691. doi: 10.3390/molecules26092691

•         Iosageanu A, Oancea A, Ilie D, Anton ED, Craciunescu O, 2021, The effect of fish bone bioactive peptides on the wound healing process: an in vitro study on keratinocytes, Romanian Biotechnological Letters, 26(3), 2692-2699. doi: 10.25083/rbl/26.3/2692.2699

•         Ilie D, Iosageanu D, Craciunescu O, Seciu-Grama AM, Sanda C, Oancea F, 2021, Free radicals scavenging, redox balance and wound healing activity of bioactive peptides derived from proteinase K-assisted hydrolysis of Hypophthalmichthys molitrix skin collagen, Food Technology and Biotechnology, (în evaluare).

Perta-CriSan, S., Ursachi, C. S., GavrilaS, S., Oancea, F., & Munteanu, F. D. (2021). Closing the Loop with Keratin-Rich Fibrous Materials. Polymers, 13(11), 1896. doi:10.3390/polym13111896

• Eggplant Peels as a Valuable Source of Anthocyanins: Extraction, Thermal Stability and Biological Activities/2021/Plants/Condurache, N.N., Croitoru, C., Enachi, E., Bahrim, G.E., Stanciuc, N., Râpeanu, G./Publicat (10, 577, https://doi.org/10.3390/plants10030577)
• Onion (Allium cepa L.) peel extracts characterization by conventional and modern methods/2021/International Journal of Food Engineering/ Constantin, O., Milea, S.A., Bolea, C.A., Mihalcea, L., Enachi, E., Copolovici, D.M., Copolovici, L., Munteanu, F., Bahrim, G.E., Râpeanu, G./Publicat (17(6), 485-493, https://doi.org/10.1515/ijfe-2020-0310)
• Glycation of whey protein isolates with xylose to improve the microencapsulation capacity of flavonoids extracted from yellow onions skins/2021/Food Chemistry/Milea, S.A., Aprodu, I., Enachi, E.,  Barbu, V., Râpeanu, G., Bahrim, G., Stanciuc, N./ În evaluare
• Microencapsulated multifunctional ingredient from aqueous flavo-noidic extract of yellow onion skins with cytocompatibility and cell proliferation properties/2021/Applied Sciences/Milea, S.A., Craciunescu, Râpeanu, G., Oancea, A., Enachi, E., O., Bahrim, G.E., Stanciuc, N./În evaluare
• Trica, B., Constantinescu-Aruxandei, D., Lukova, P. Michaud,  P., Dellatre, C., Oancea F.,  (2021) Application of biopolymers from marine environment for terrestrial crop treatment, Polymers, in evaluare

Presentations

1. The 9th Edition of the Scientific Conference of the Doctoral Schools of Dunarea de Jos University, Galati/National/Impact of wall materials on physicochemical properties and stability of eggplant peels anthocyanin hydrogels/2021, Nina-Nicoleta Condurache (Lazar), Gabriela Elena Bahrim, Nicoleta Stanciuc, Gabriela Râpeanu
2. The 9th Edition of the Scientific Conference of the Doctoral Schools of Dunarea de Jos University, Galati/National/Ice cream enriched with microencapsulated bioactive compounds from eggplant peels and LAB/2021, Nina-Nicoleta Condurache (Lazar), Gabriela Elena Bahrim, Nicoleta Stanciuc, Gabriela Râpeanu
3. The 9th Edition of the Scientific Conference of the Doctoral Schools of Dunarea de Jos University, Galati/National/Effect of glycosylation with xylose on the ability of whey proteins to encapsulate the flavonoids from yellow onions skins/2021, Stefania Adelina Milea, Iuliana Aprodu, Elena Enachi, Gabriela Râpeanu, Gabriela Elena Bahrim, Nicoleta Stanciuc
4. The 9th Edition of the Scientific Conference of the Doctoral Schools of Dunarea de Jos University, Galati/National/Functional ingredients based on yellow onion skins extract and whey bioactive peptides for use in the food industry/2021, Stefania-Adelina Milea, Liliana Mihalcea, Aida Mihaela Vasile, Nicoleta Stanciuc, Gabriela Râpeanu, Gabriela Elena Bahrim
5. Nutricon 2021, Ohrid, Macedonia/International/Multifunctional ingredients for food, nutraceutical and pharmaceuticals applications exploting the health benefits of yellow onion skins phytochemicals and lactic acid bacteria through co-microencapsulation/2021, Nicoleta Stanciuc
6. 10^th Conference Agriculture for Life, Life for Agriculture,  / International/ Effects of extracts from Passiflora caerulea leaves treated with a Trichoderma biostimulant consortium on lactic acid bacteria, Elena Utoiu, Iulia Raut, Florin Oancea

A Joint CDI Programme of the partners of the complex project 10PCCDI PRO-SPER has been developed whose objectives are:

• Stimulating innovation by  participating in at least two consortium partners in European project competitions (Horizon Europe – European Partnership for a Europe with Biocircular Value Chains, Innovation Ecosystems), transnational (ERA.NET, Eurostar / Eureka), Structural and National Funds to Finance Innovation, to scale up  their technologies developed up to TRL4 through the projects of the complex PRO-SPER project,
• Developing a long-term partnership for the provision of scientific and technological services, at the intersection of common bio-nano-technology concerns, in the priority area of the circular bioeconomy and for the strengthening of the component CDI institutions, through the joint undertaking of actions aimed at developing research infrastructure and the newly committed one, to increase labor productivity
• Harnessing the new knowledge and  skills acquired in the PRO-SPER complex project through joint participation in European projects (Horizon 2020, ERC and Pillar 2, Clusters 5 Climate, Energy and Mobility, and 6, Food, Bioeconomy, Resources, Natural, Agriculture and Environment), Transnational (ERA.NET) and National (Partnership Solutions)
• Communication and dissemination of their knowledge and research results obtained under the PRO-SPER project to increase their impact on target sectors.

The partners agree to maintain the newly created posts created by project 10 PCCDI PRO-SPER for at least another two years.

The specific objectives of the PRO-SPER complex project have been achieved:

• Implementing a common agenda for R&D and innovation, through which the consortium's competencies and resources are synergistically used to provide innovative solutions intended to close the value chains in the bioeconomy;
• Strengthening  their component CDI institutions by providing cheques for the recovery of research infrastructure and human resources and the transfer of knowledge in the field of the bioeconomy;
• Developing a long-term partnership for the provision of scientific and technological services in the priority field  of the circular bioeconomy  and mutually beneficial collaboration with economic operators, including for the joint exploitation of project results;
• Participation in joint European (H2020, JU-BBI) and transnational (ERA.NET, Eurostar /Eureka) common competitions, at least two partners in the consortium, for the scale of   technologies developed up to TRL4 level through component projects and/or the exploitation of newly acquired knowledge;
• Promoting  excellence and smart specialization by coagulating niche areas at the intersection of common nano-biotechnology concerns and synergistically integrating several interconnected innovative solutions;
• Widespread dissemination of their knowledge and  research results obtained under the PRO-SPER project to ensure their superior exploitation;
• Use of Responsible Research & Innovation tools to engage all stakeholders in permanent dialogue to facilitate fine-tuning component project activities according to socio-economic needs.

               A joint research agenda has been implemented. Closing the loop of the value chains through bio-nano-technological solutions has been defined by series of the project's scientific coagulation axes,  with significant scientific and socio-economic impact. These coagulation axes were the basis for increasing the institutional capacity of PRO-SPER partners. These coagulation axes are:

• Intensification of the extraction and/or formation of biologically active compounds in agro-industrial co/sub-products, including by the use of enzymes acting on the lignocellulosic matrix in combination with natural eutectic solvents
• Development of natural eutectic solvents  (NofDES), as well as the aqueous ternary systems they form, for the selective extraction of hard-to-dissolvable components (e.g., lignin), which reduce the accessibility of active ingredients in by-products;
• Production of chitosan  from  by-products from the cultivation of edible mushrooms (macromycets), with more excellent uniformity in terms of degree and pattern of deacetylation and molecular mass, followed by the grafting of flexible imin units on uniform chitosan chains with the production of glycodinamers;
• Obtaining bio-active peptides  from different sources (proteins from fungi, whey, fish by-products), etc., used for high-value products
• Implementation of high-yield experimentation technology  (HTT), involving programmed experiments, with the simultaneous variation of several experimental parameters, and/or the parallel conduct of experiments and/or (bio)tests necessary for the development and optimization of new technological solutions / new products, which lead to the acceleration of CDI activities.

               The joint research agenda was also reflected in the development of the  CDI Joint Programme, which linked the partners' institutional development plan. The two main research directions of the CDI PRO-SPER Joint Programme, namely: (i)  the sustainable intensification of the production of bioresources and (ii)  the "intelligent" use of bioresources, are aimed at countering the main weaknesses of the Romanian bioeconomy, low productivity, and low profitability.