Nanocellulose 3-D structures for regenerative medicine (CELL-3D)

  • Funding: Romanian National Authority for Scientific Research and Innovation, CNCS - UEFISCDI
  • Programme: P4 Basic and Frontier Research - Exploratory Research Projects PN-III-P4-ID-PCE-2016-0431
  • Project number: PN-III-P4-ID-PCE-2016-0431
  • Research domain - Engineering Sciences
  • Project title: Nanocellulose 3-D structures for regenerative medicine (CELL-3D)
  • Financing contract: 148/2017
  • Total funding of the contract: 850.000 RON
  • Funding from the State Budget: 850.000 RON
  • Project duration: 30 months
  • Period of project implementation: 12/07/2017 - 31/12/2019
  • Contractor: National Institute for Research & Development in Chemistry and Petrochemistry - ICECHIM, Bucharest
  • Project Manager: Panaitescu Denis Mihaela - panaitescu@icechim.ro

Regenerative medicine and tissue engineering may offer new hope of life in case of loss or malfunction of a tissue or organs. One of the key elements in tissue engineering is the three-dimensional (3D) scaffold which provides structural support for cell attachment, proliferation and differentiation. Biopolymer scaffolds are preferred in tissue engineering for avoiding the issues related to donor immune rejection and pathogen transfer. Cellulose, the most abundant natural polymer on earth, is an almost inexhaustible source of valuable materials and has gained high interest in the form of nanocellulose (NC). NC stands out for its high crystallinity and mechanical strength, good biocompatibility, high water-holding capacity and large surface area and it has been intensively studied for medical applications because it does not cause toxic or allergic side effects in contact with living tissue. 3D scaffolds should possess a network of interconnected pores to permit cell migration, diffusion of nutrients and clearance of wastes, they must be able to support cell adhesion and promote cell growth and they must have balanced stiffness-toughness properties to support and transfer of loads. To meet all these requirements in porous 3D scaffolding, CELL-3D has as main objective to design new 3D nanocellulose structures that can be applied in regenerative medicine.

CELL-3D has as main objective to design new 3D nanocellulose structures for tissue engineering. The new 3D structures should meet the required chemical, mechanical and structural properties needed in regenerative medicine. To address the overall objective of this project, several specific objectives will be considered: (1) understanding the influence of the surface modification of nanocellulose on the 3D structures, (2) understanding the influence of composition and processing parameters on the properties of 3D structures; (3) characterization of the properties and morphology of nanocellulose 3D structures in different environments. 

The 3D nanocellulose structures will be obtained by new methods based on the understanding of the relationship between structure, processing parameters and properties of nanocellulose 3D scaffolds. The results will be disseminated by articles and communications.

Panaitescu Denis

Vuluga Zina

Corobea Mihai

Frone Adriana

Chiulan Ioana

Nicolae Cristian

Gabor Raluca

Ficai Anton

Trusca Roxana

Ghiurea Marius

Bogdan Trica

Ninciuleanu Claudia

  1. Synthesis and characterization of 3D structures starting from different types of nanocellulose (NC) using surface functionalization. Understanding the mechanisms of 3D structures formation;
  2. Synthesis and characterization of 3D structures from nanocellulose - polymer composites. Analysis of process conditions in relation to nanocomposites structure and properties;
  3. Flexible 3D structures obtained by new techniques. Optimizing the process and elaboration of the model.

Articles

1. Adriana Nicoleta Frone, Denis Mihaela Panaitescu,* Cristian Andi Nicolae, Augusta Raluca Gabor, Roxana Trusca, Design of nanocellulose 3D structures for biomedical applications by functionalization and cross-linking, Submitted to Applied Surface Science, november 2018

2. Sorin Vizireanu, Denis Mihaela Panaitescu,* Cristian Andi Nicolae, Adriana Nicoleta Frone, Ioana Chiulan, Maria Daniela Ionita, Veronica Satulu, Lavinia Gabriela Carpen, Simona Petrescu, Ruxandra Birjega, Gheorghe Dinescu, Cellulose defibrillation and functionalization by plasma in liquid treatment, Scientific Reports(2018) 8, 15473

3. Denis Mihaela Panaitescu, Eusebiu Rosini Ionita, Cristian Andi Nicolae, Raluca Augusta Gabor, Maria Daniela Ionita, Roxana Trusca, Brindusa-Elena Lixandru, Irina Codita, Gheorghe Dinescu, Poly(3-hydroxybutyrate) modified by nanocellulose and plasma treatment for packaging applications, Polymers (2018) 10, 1249

4. Denis Mihaela Panaitescu*, Adriana Nicoleta Frone, Ioana Chiulan, Cristian Andi Nicolae, Roxana Trusca, Marius Ghiurea, Raluca Augusta Gabor, Mona Mihailescu, Angela Casarica, Irina Lupescu, Role of bacterial cellulose and poly(3-hydroxyhexanoate-co-3-hydroxyoctanoate) in poly(3-hydroxybutyrate) blends and composites, Cellulose (2018) 25, 5569–5591

5. Adriana Nicoleta Frone, Denis Mihaela Panaitescu,*, Ioana Chiulan, Cristian Andi Nicolae, Angela Casarica, Raluca Augusta Gabor, Roxana Trusca, Celina Maria Damian, Violeta Purcar, Elvira Alexandrescu, Paul Octavian Stanescu, Surface treatment of bacterial cellulose in mild, eco-friendly conditions, Coatings (2018) 8, 221

6. Ioana Chiulan, Adriana Nicoleta Frone, Calin Brandabur, Denis Mihaela Panaitescu, Recent Advances in 3D Printing of Aliphatic Polyesters, Bioengineering (2017) 5(1) 2.

  

Conferences 

1. Denis Mihaela Panaitescu, Adriana Nicoleta Frone, Ioana Chiulan, Cristian Nicolae, Raluca Gabor, Elvira Alexandrescu, Angela Casarica, Design of nanocellulose aerogels by functionalization and cross-linking, 11th International conference on Advanced Nanomaterials, Aveiro, Portugal, 18-20 July 2018

2. Adriana Nicoleta Frone, Ioana Chiulan, Raluca-Augusta Gabor, Elvira Alexandrescu, Cristian-Andi Nicolae, Denis Mihaela Panaitescu, 3D structures from cross-linked nanocellulose, 6th Portuguese Young Chemists Meeting - PYCheM2018, Setubal, 15-18 May 2018

3. Adriana Nicoleta Frone, Denis Mihaela Panaitescu, Ioana Chiulan, Raluca Augusta Gabor, Cristian Andi Nicolae, Thermal behavior of biodegradable polyesters based membranes, 12th European Symposium on Thermal Analysis and Calorimetry, Brasov, Romania, 27-30 August 2018

4. Denis Mihaela Panaitescu, Adriana Nicoleta Frone, Ioana Chiulan, Cristian Andi Nicolae, Augusta Raluga Gabor, Madalina Calarasu, Sergiu Stoian, Roxana Trusca, Angela Casarica, New 3D structures from nanocellulose and biopolymers, XXXVth National Conference of Chemistry, Caciulata, Romania, 2-5 October 2018

5. Denis Mihaela Panaitescu, Adriana Nicoleta Frone, Madalina Calarasu, Sergiu Stoian, Porous structures from nanocellulose and biopolymers for biomedical application, 9th International Conference on Biopolymers and Polymer Sciences, Bucharest, Romania, 19-21 November 2018

6. Sergiu Stoian, Adriana Nicoleta Frone, Denis Mihaela Panaitescu, Mona Mihailescu, Roxana Trusca, Cristian Andi Nicolae, Augusta Raluga Gabor, Physico-chemical characterization of surface modified cellulose, Sixth edition of the International Colloquium ‘Physics of Materials’, Bucharest, Romania, 15-16 November 2018

7. D. M. Panaitescu, A. N. Frone, I. Chiulan, R. A. Gabor, C.A. Nicolae, M. Ghiurea, D. Florea, C. M. Ninciuleanu, Surface functionalization of nanocellulose for biomedical applications, The XIIIth International Symposium "Priorities of Chemistry for a Sustainable Development – PRIOCHEM”, Bucharest, 25 - 27 October 2017